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At-line Detection of Viral and Bacterial Contaminants in Mammalian Cell Culture Using High Affinity Probes and Label-free Single-cell Analysis

The project goals are to design and implement a combination of laser force cytology cell analysis and sorting and capillary electrophoresis (CE)-based methods to screen for viral and bacterial contamination, along other process parameters.
Categories
Assays
Equipment and Supplies
Project status
100% Completed

Solution

Performance Period: 4/1/2018 to 3/31/2021

We propose to design and implement a combination of laser force cytology cell analysis and sorting and capillary electrophoresis (CE)-based methods to screen for viral and bacterial contamination, along other process parameters. Detection will be accomplished using a rapid, gel-free analysis platform (“micelle-ELFSE”) and high affinity unnatural nucleic acid probes. A laser force cytology system will be developed for label-free detection of virus and bacteria. The two systems will be integrated with the goal of at-line or in-line detection along with assessment of final product purity. 

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Project Lead

Carnegie Mellon University

Carnegie Mellon University

Participating Organizations

Genentech

Genentech

LumaCyte

LumaCyte

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