This project aims to implement a rapid, reliable electrophoretic assay of critical quality attributes (CQA) of mRNA lipid nanoparticles (LNPs) to be used in mRNA vaccines.
The team will also subject LNPs to various manufacturing and storage insults and compare CE assay readout with that from state-of-the-art bioassays of mRNA quantity, delivery efficacy, and immunogenicity.
Optimize the assay so it can be used to rapidly quantitate mRNA from LNPs
Distinguish between folded and unfolded versions of mRNA to help detect damage
Method could be used to routinely test formulated LNPs at various stages of their manufacture and/or storage
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