Advanced FISH Assay and Mechanism Hybrid Surrogate to Improve mRNA Vaccine Potency Assessment and Prediction

The objective of this project is to develop and validate single-molecule RNA-fluorescence in situ hybridization (smFISH), mechanistic & hybrid surrogate model, and PATs for mRNA delivery and translation processes to ensure vaccine efficacy.

Project status

50% Completed

Industry Need

The COVID-19 pandemic promoted the rapid development of mRNA-based vaccines targeting a wide range of infectious diseases.
The ongoing emergence of various virus variants creates the critical need for quality control and assurance (QC/QA) to ensure mRNA vaccines have high potency (i.e., a quantifiable biological response elicited by the antigen or vaccine product).

Solution

Northeastern University aims to provide a multiplexed potency assay and a delivery process mechanism surrogate that can be useful for commercially QC/QA screening multivalent mRNA vaccines encoding multiple proteins of variant strains.

Updates, Related Publications, and Deliverables

Not yet a member? Learn more about which level of NIIMBL membership is right for you and your organization.

Project Lead

Northeastern University