Close this search box.

Advanced FISH Assay and Mechanism Hybrid Surrogate to Improve mRNA Vaccine Potency Assessment and Prediction

The objective of this project is to develop and validate single-molecule RNA-fluorescence in situ hybridization (smFISH), mechanistic & hybrid surrogate model, and PATs for mRNA delivery and translation processes to ensure vaccine efficacy.
Process control
Project status
38% Completed

Industry Need

  • The COVID-19 pandemic promoted the rapid development of mRNA-based vaccines targeting a wide range of infectious diseases. 
  • The ongoing emergence of various virus variants creates the critical need for quality control and assurance (QC/QA) to ensure mRNA vaccines have high potency (i.e., a quantifiable biological response elicited by the antigen or vaccine product). 


Northeastern University aims to provide a multiplexed potency assay and a delivery process mechanism surrogate that can be useful for commercially QC/QA screening multivalent mRNA vaccines encoding multiple proteins of variant strains.  

Updates, Related Publications, and Deliverables

  • Login to the NIIMBL member portal for access to project updates, related publications, and deliverables. 

Not yet a member? Learn more about which level of NIIMBL membership is right for you and your organization.

Project Lead

Northeastern University

Northeastern University

Join Our Mailing List

MailChimp NIIMBL Mailing List Signup
Organization Type
How did you first hear about NIIMBL?