Design and implement a combination of laser force cytology cell analysis and sorting and capillary electrophoresis (CE)-based methods to screen for viral and bacterial contamination, along other process parameters.
Laser force cytology gives virus detection sensitivity equal to or exceeding that of cytopathic effect (CPE) assays.
LFC instrument installed for detection of MMV in CHO cells – also found sensitivity equal to or exceeding CPE using statistical methods developed in the project.
Micelle-tagging electrophoresis (MTE), using peptide nucleic acid (PNA) probes, detected viral DNA / RNA down to 10 fM in a 10-minute run.
Publication: P.R. Hayes. T.M. Przybycien, J.W. Schneider. “Viral adventitious agent detection using laser force cytology: Intrinsic cell property changes with infection and comparison to in vitro testing,” submitted to Biotech. Bioeng. (2021)
Direct, PCR-less Tagging of Viral RNA using Micelle Tagging Electrophoresis (MTE) – June 19, 2019
Allows for process troubleshooting and screening of raw materials, cell banks and bulk harvest materials
Rapid at-line detection of viral contaminants
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Carnegie Mellon University
Genentech, Inc.
LumaCyte
