Performance Period: 9/1/2020 to 2/28/2022
We propose to develop an innovative technology platform for cell therapy manufacturing that can accommodate all the necessary unit operations, including separation of cell phenotypes, activation and transduction, and expansion of purified phenotypes. The proposed device is a flow system based on existing membrane technology, allowing facile scaling and sufficient versatility for provision of nutrients and oxygen, as well as convective enhancement of vector transport for transduction. A unique feature is the integration of tailorable soft materials that will provide optimal microenvironments to facilitate and control cell capture, release, manipulation, and promotion of desired functions.
The initial project period proposed here will be devoted to device construction, optimization of cell microenvironments for different operations, and integration of different unit operations, including testing in realistic manufacturing environments in industry partner labs. We expect that the proposed technology will be enabling for many of the steps necessary for current and emerging forms of cell therapy.
Develop an innovative technology platform for cell therapy manufacturing that can accommodate all the necessary unit operations, including separation of cell phenotypes, activation and transduction, and expansion of purified phenotypes
Integration of tailorable soft materials that will provide optimal microenvironments to facilitate and control cell capture, release, manipulation, and promotion of desired functions
Bomb, K., LeValley, P. J., Woodward, I. R., Cassel, S. E., Sutherland, B. P., Bhattacharjee, A., Yun, Z., Steen, J., Kurdzo, E., McCoskey, J., Burris, D., Levine, K., Carbrello, C., Lenhoff, A. M., Fromen, C. A., & Kloxin, A. M. (2023). Cell Therapy Biomanufacturing: Integrating Biomaterial and Flow-Based Membrane Technologies for Production of Engineered T-Cells. Advanced Materials Technologies, 8(6), 2201155. https://doi.org/10.1002/admt.202201155
Lopez Ruiz, A., Slaughter, E. D., Kloxin, A. M., & Fromen, C. A. (2024). Bridging the gender gap in autoimmunity with T-cell-targeted biomaterials. Current Opinion in Biotechnology, 86, 103075. https://doi.org/10.1016/j.copbio.2024.103075
Login to the NIIMBL member portal to access more, including:
Not yet a member? Learn more about which level of NIIMBL membership is right for you and your organization.
University of Delaware
Merck Sharp & Dohme LLC
MilliporeSigma/EMD Serono
