Continuous Countercurrent Spiral Chromatography (CCSC) – Feasibility, Demonstration and Commercialization
Development of a new device format called the spiral sorter into process development within the CCTC framework.
Categories
Proteins/ Antibodies
Drug substance
Project status
100% Completed
Industry Need
Protein A chromatography is effective for mAb recovery and impurity removal without pretreatment. However, it has drawbacks like high resin costs, low productivity, and complex column packing and cleaning. Advances in cell culture have increased mAb titers, shifting bottlenecks to downstream operations and highlighting the need for innovation in mAb capture.
Solution
The project goal is to develop and implement a
novel Protein A chromatography system using a 3D printed spiral sorter specifically designed for long-term continuous operation for use as part
of an integrated continuous biomanufacturing process.
Outputs/Deliverables
Two geometries were evaluated for the spiral sorter design.
Able to achieve separation efficiencies of 75-85% at conditions that are realistic to CCSC operation.
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