Currently, liquid chromatography coupled with mass spectrometry is the preferred technique for the simultaneous analysis of metabolites and nutrients. Due to the size and complexity of the instrumentation, analyses are done off-line in a centralized lab by highly trained personnel. Such analyses require lengthy sample preparation steps, and relatively large sample volumes (milliliters) making them incompatible with early process development studies in microbioreactors or near real-time bioreactor monitoring.
We proposed the development of a compact microchip-based capillary electrophoresis-high pressure mass spectrometer (CE-HPMS) platform capable of monitoring both cation and anion metabolites and growth media constituents in near real time.
Develop a compact microchip-based capillary electrophoresis-high pressure mass spectrometer (CE-HPMS) analyzer for near real-time monitoring of the nutrients and cell metabolites involved in the biomanufacturing process.
Login to the NIIMBL member portal to access more, including:
Not yet a member? Learn more about which level of NIIMBL membership is right for you and your organization.
University of North Carolina, Chapel Hill
908 Devices, Inc.
Bristol-Myers Squibb
MilliporeSigma/EMD Serono
North Carolina State University
