Chromatographic technology that combined two orthogonal separation modalities exists for viral vectors and vaccines, however not for therapeutic proteins.
Apply the existing purification paradigm from viral vectors and vaccines to proteins.
Single-use cartridge for capturing antibody fragments and host cell proteins from Protein A elutes in flow-through mode
Optimize the mixed-mode ligands and the pore diameter of SEMM-silica beads to achieve efficient capture of the target species and high whole-mAb yield
Optimize particle size of silica beads and cartridge packing to enable fast flow, thus achieving fast processing with minimal pressure drops
Mixed-mode silica resins packed in cartridges for single-use applications, thus eliminating the need for cleaning and sanitization in place
LeBarre, J. P., Chu, W., Altern, S. H., Kocot, A. J., Bhandari, D., Barbieri, E., Sly, J., Crapanzano, M., Cramer, S. M., Phillips, M., Roush, D., Carbonell, R., Boi, C., & Menegatti, S. (2024). Mixed-mode size-exclusion silica resin for polishing human antibodies in flow-through mode. Journal of Chromatography A, 1720. https://doi.org/10.1016/j.chroma.2024.464772
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North Carolina State University
LigaTrap Technologies, LLC.
Merck Sharp & Dohme LLC
MilliporeSigma/EMD Serono
Rensselaer Polytechnic Institute