Spectro-acoustic Process Analytical Technology for Continuous Manufacturing of Coronavirus Vaccine

This project aims to demonstrate Raman spectroscopy and acoustic microfluidics in enabling PAT to detect and quantify virus-like particles and attenuated live viruses to aid the continuous manufacturing of vaccines for future coronavirus.
Categories
Active Immunization Countermeasures

Industry Need

  • Viral epidemics and pandemics require rapid development and manufacturing of vaccines to limit the spread of the disease and minimize loss of life.  
  • Rapid production of vaccines requires standardization processes for controlling the quality of the product. 
  • Current methods for determining the formulation of attenuated live viruses and virus-like particles are limited because they cannot provide real-time information. 


Solution

Process analytical technology aims to improve biomanufacturing by providing real-time information about the product, enabling real-time release. This team will use of spectroscopy techniques for continuous in-process monitoring of virus particles and establish the basis for a standardized detection method. Doing so will establish a standard method that follows FDA guidance for future manufacturing of vaccines and address the need for uniform and controlled platform approaches. 

Outputs/Deliverables

  • Design and produce prototype acoustofluidic device
  • Couple Raman spectrometer to acoustofluidic device and optimize conditions and characterize viral media for high-quality Raman spectra
  • Validate the deep learning algorithm for analyzing virus particles and build a graphical user interface

Impacts

Successful completion of the project will lead to a spectro-acoustic process analytical technology that enables real-time quantification of virus particles necessary for continuous manufacturing of future vaccines towards coronaviruses.

Publications

Additional Project Information (Members Only)

Login to the NIIMBL member portal to access more, including: 

  • Updates
  • Value Proposition
  • Related Publications
  • Deliverables

Not yet a member? Learn more about which level of NIIMBL membership is right for you and your organization.

Project Lead

Purdue University

Purdue University