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Use of Metabolic Flux Analysis (MFA) to Expedite Media Selection and Optimization of CHO

Development of a diagnostic kit to integrate metabolic flux analysis (MFA) that can be used to identify best CHO media composition.
Proteins/ Antibodies
Drug substance
Project status
100% Completed

Industry Need

  • Current approaches to identify appropriate media formulations for CHO cell factories are lengthy, costly, and labor-intensive. 
  • Industry would benefit from improved media selection processes and accelerated time to production


MilliporeSigma partnered with Metalytics, Inc. to integrate metabolic flux analysis (MFA) into a diagnostic kit that can be used to identify best media composition quickly and effectively for a given CHO cell line.  

To do this MilliporeSigma and Metalytics:  

  • Stratified a diverse set of CHO lines into distinct groups, each characterized by its own set of nutritional requirements 
  • Used MFA characteristics of these CHO clonal groups to rationally design and optimize media and process for each group 
  • Created a diagnostic toolkit that can be used by end users to pair each clone with a “best fit” media formulation that will maximize metabolism of raw ingredients into pharmaceutical product 

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Project Lead

MilliporeSigma/EMD Serono

MilliporeSigma/EMD Serono

Participating Organizations



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