The cost of a viral contamination event can be as much as $100 million due to lost sales and product, in addition to shortage of drug supply to patients.
A new, more rapid but still broad, sensitive detection of virus method to use in viral vaccine production.
General test for viral infectivity in cultured cells without requiring the use of specific probes
Cultured cells analyzed for infectivity in real time (hundreds of cells analyzed in less than 5 minutes)
Reduce the amount of time required by an assay to detect virus in cultured cells thus lowering the cost of a contamination event and resulting in less drug supply lost
By implementing a label-free, in-process infectivity assay using Laser-Force Cytology (LFC) combined with micelle-tagging electrophoresis (MTE) and predictive infection modeling, an organization will reduce viral vaccine production cycle time by 1–3 days and improve detection sensitivity by up to 10×, enabling earlier and more accurate infection monitoring.
Cells isolated from carriers during batch production of virus with Radiance-measured cell properties showing good correlation with infection extent, virus titer Several model virus quantitated at sub-fM levels using capillary electrophoresis Model for batch production of measles virus in Vero cells predicts significant transients in behavior and can be used to parse Radiance-measured cell property distributions
McCracken, R., Al-Nazal, N., Whitmer, T., Yi, S., Wagner, J. M., Hebert, C. G., Lowry, M. J., Hayes, P. R., Schneider, J. W., Przybycien, T. M., & Mukherjee, M. (2022). Rapid In-Process Measurement of Live Virus Vaccine Potency Using Laser Force Cytology: Paving the Way for Rapid Vaccine Development. Vaccines, 10(10). https://doi.org/10.3390/vaccines10101589
Hebert, C., Schneider, J. W., Lowry, M., Le Roux, D., Hart, S., Mccracken, R., Al-Nazal, N., Yi, S., Whitmer, T., Mukherjee, M., Westrek, B., Przybycien, T. M., Samuel, S., & Hayes, P., PC3.1-127 Specific Detection of Infected Cells using Optical Drag-Tags in Laser Force Cytology, NIIMBL National Meeting, Washington, DC, July 14, 2021.
Schneider, J., Presenter, PC3.1-127: Specific Identification of Infected Cells using Laser Force Cytology and High Affinity Probes in Isotachophoresis, NIIMBL National Meeting, Washington, D.C., July 27, 2022.
Specific Detection of Infected Cells using Optical Drag-Tags in Laser Force Cytology (PC3.1-127), NIIMBL Member Forum, Virtual, February 24, 2022.
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Carnegie Mellon University
LumaCyte
Merck Sharp & Dohme LLC
Rensselaer Polytechnic Institute
