Current T cell manufacturing methods, adapted from other medical uses, face challenges like high costs, safety concerns, and inefficiencies, especially for leukemia patients. Automation is being developed to address these issues, but its success is still uncertain.
The proposed solution involves developing a closed-loop perfusion circuit for T cell engineering and expansion using two bioreactors: a small one for selection, activation, and transduction (Stage 1), and a larger one for expansion (Stage 2).
Demonstration of two different-size bioreactors using 3D printing.
Method to immobilize antibodies to bioreactor surface.
Demonstration of activation of T-cells in the 3D printed single use bioreactor.
Demonstration of bioreactor as a pre-filter for T cell selection and purification.
Demonstration of the perfusion-based beads-free bioreactor has potential to form an automated closed-loop system for cost-effective T cell manufacturing.
The goal of the project is to develop a superior process / platform for selection, activation and lentiviral vector transduction of human T-cells using perfusion bioreactor system
The project will enable continuous processing of T-cells in a closed environment and reduce process costs
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Southwest Research Institute
