Isomer Level Identification and Quantification of N-Linked Glycans by LC-SLIM-QTOF-MS

This project at North Carolina State University will be led by David C. Muddiman. This project is part of a larger small-group study to evaluate the variability associated with operator-associated variability in measuring released glycans from a monoclona
Categories
Assays
Equipment and Supplies
Data
Project status
100% Completed

Industry Need

  • Enhanced and/or standardized ability to measure and quantitatively evaluate the glycan profile of a reference monoclonal antibody 

Solution

NC State University will procure an integrated LC-SLIM-QTOF-MS from Agilent Technologies. This approach will be used to measure and quantitatively evaluate the glycan profile of a reference monoclonal antibody using glycan standards as well as the glycan profile of a reference monoclonal antibody where the release was performed at a central laboratory. 

Outputs/Deliverables

  • Measured and reported peak areas for glycans detected from standard mixtures
  • Measured and reported peak areas for glycans detected from NIST mAb
  • Quantitated G0F in NIST mAb using three different forms of chemical quantitation



Impacts

Upon project completion, the the Agilent/MobilIon LC-SLIM-QTOF-MS and GlycoHunter will be available on a priority basis for NIIMBL-related technology and workforce activities, within any necessary constraints of the host institution, to be negotiated with NIIMBL.

Publications

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Project Lead

North Carolina State University

North Carolina State University