Real-Time, Absolute Quantitation of Critical Bioprocessing Metabolites with the AI-powered PyxisTM Metabolomics Platform

Evaluate and validate Matterworks’ Pyxis metabolomics platform to improve bioprocessing. Pyxis provides rapid, absolute quantitation of ~150 metabolites across intracellular pathways. Also explore use of Pyxis for early detection/monitoring of glycoforms.
Categories
Drug substance
Process control
Project status
100% Completed

Industry Need

Methods and/or platforms for improving bioprocessing consistency and efficiency.

Solution

The goal of this project is to evaluate and validate Matterworks’ Pyxis metabolomics platform to improve bioprocessing consistency and efficiency.  

  • A proprietary consumable kit that can fit into current LCMS workflows and automated data processing will enable the analysis of cell lysates and media. 
  • Pyxis provides rapid, absolute quantitation of ~150 metabolites across intracellular pathways that impact cell growth, protein production and processing, and glycosylation.  
  • Project input from partners will provide important feedback to optimize Pyxis product specifications and help guide future development for at-line monitoring.  

Outputs/Deliverables

  • Demonstrated that Pyxis could be used to track the rise and fall of the absolute concentration of key metabolites in both the media samples and cells over the typical operating cycle of a bioreactor.  
  • Demonstrated that the absolute concentration data from Pyxis is comparable to that obtained using traditional LC-MS techniques, meaning that for 80% of the 30 analytes it delivered concentrations which were within 30% the accepted standards. Unlike the orthogonal technique, however, Pyxis concentrations can be obtained rapidly by non experts.
  • Demonstrated that there were some observed concentration differences between typical bioreactors and those in which there were forced deviations – but did not fully demonstrate that such changes could be used to predict outcome.

Impacts

Develop Pyxis as a proprietary consumable kit that fits seamlessly into current LCMS workflows and automated data processing enable the analysis of cell lysates and media

Explore using Pyxis for early detection and monitoring of glycosylation activity impacting glycoform profiles and process excursions

Publications

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Project Lead

Matterworks, Inc.

Matterworks, Inc.

Participating Organizations

Eli Lilly and Company

Eli Lilly and Company

Sartorius Stedim

Sartorius Stedim