Currently, there is no reliable scale-down model for perfusion culture to be used for cell line development and process optimization. Existing perfusion culture using hollow fiber membranes (ATF or TFF) is too difficult to maintain.
We aim to develop a fully automated smallscale (<500 mL) membrane-less perfusion bioreactor system for CHO cells at very high concentration (50–100E+6 cells/mL) using a novel cell retention device based on inertial sorting.
Jeon, H., Kwon, T., Yoon, J., & Han, J. (2022). Engineering a deformation-free plastic spiral inertial microfluidic system for CHO cell clarification in biomanufacturing. Lab on a Chip, 22(2), 272-285. https://doi.org/10.1039/d1lc00995h
Jeon, H., Kwon, T., Yoon, J., & Han, J., Biomanufacturing Scale CHO Cell Clarification Using Hard Plastic Spiral Inertial Microfluidic Device, MicroTAS 2021, Virual, October 20, 2021.
Kwon, T., Choi, K., & Han, J. (2021). Separation of Ultra-High-Density Cell Suspension via Elasto-Inertial Microfluidics. Small, 17(39). https://doi.org/10.1002/smll.202101880
Rapid perfusion culture process development with a simple and low cost cell retention device
Fully automated small-scale perfusion culture using a membrane-less cell retention device
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Massachusetts Institute of Technology
EMD Millipore Corporation
Massachusetts Life Sciences Center
Merck Sharp & Dohme LLC
Sartorius Stedim
Whirlcell LLC.
