NIPTE and Pfizer have teamed up to demonstrate the ability of TG-RS to reduce fluorescence and improve SNR of key metabolites in bioreactions. The superior SNR will enable the development and comparison of PLS and pure component models for the quantification of these metabolites during bioreactions of industrially relevant organisms.
TG-RS is expected to significantly improve the ability to monitor chemical conditions in bioreactors.
Demonstrate that combining TG-RS and pure component modeling improves bioreaction monitoring capability while reducing calibration resource burden in a system ready for industrial deployment
The real-time data collected during reactor monitoring can be used as an input in a control strategy to maximize product yield and quality while minimizing inefficiencies that lead to product failure and loss of profit.
Use the real-time data collected during reactor monitoring as an input in a control strategy to maximize product yield and quality while minimizing inefficiencies that lead to product failure and loss of profit
Broad acceptance and implementation of TG-RS as an industry standard technology for bioreaction monitoring
Demonstrate the suitability of TG-RS for regulators as an appropriate process analytical technology (PAT) system
Work will demonstrate the suitability of TG-RS for regulators as an appropriate process analytical technology (PAT) system
Create broad acceptance and implementation of TG-RS as an industry standard technology for bioreaction monitoring
Time-gated Raman spectroscopy (TG-RS) delivers a superior Signal-to-Noise Ratio (SNR) by isolating Raman signals from fluorescence, allowing for more precise tracking of key metabolites compared to traditional methods. By enabling "calibration-free" models, this technology can reduce the time required for model development and calibration by an estimated 50% to 70% compared to labor-intensive partial least squares (PLS) approaches. This enhanced spectral clarity directly mitigates the risk of batch failures in complex bioreactions, leading to higher yield consistency and a significant reduction in the thousands of dollars lost during a single failed industrial run.
Anderson, Carl., Presenter, NIIMBL National Meeting conference, Washington D.C., June 26, 2025.
Anderson, C., Conference Participant, PC 5.2-134: Enhancing Analytical Sensitivity in Upstream Bioreactions Using Time-gated Raman Spectroscopy, NIIMBL National Meeting, Washington, D.C., June 26, 2025.
Anderson, Carl., Presenter, NIIMBL National Meeting conference, Washington D.C., June 25, 2025.
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National Institute for Pharmaceutical Technology and Education, Inc (NIPTE)
Pfizer, Inc.
