MilliporeSigma partnered with Metalytics, Inc. to integrate metabolic flux analysis (MFA) into a diagnostic kit that can be used to identify best media composition quickly and effectively for a given CHO cell line.
To do this MilliporeSigma and Metalytics:
Create a guided metabolic profiling toolkit to streamline identification of optimal CHO cell media formulations.
Reduced media development screening costs by using a predicted "best fit" medium formulation for CHO cell clonal groups
Furthered understanding of CHO cell metabolism variability across a wide selection of cell lines
Increased ability to use this knowledge to improve media and develop better culture processes, resulting in faster and better methods to improve productivity of recombinant proteins etc. in CHO cultures
Demonstrated the utility of MFA as a tool to accelerate media optimization
Determined that nearly all clones regardless of genetic background have different metabolic profiles
By implementing a metabolic flux analysis (MFA)-based diagnostic kit for media optimization, organizations could reduce CHO media development timelines by approximately 3 months, enabling faster upstream processes and lowering costs– driving significant gains in productivity and efficiency across biomanufacturing.
Metabolic Flux Analysis (MFA) was added to services MilliporeSigma can offer for media optimization for CHOZN and CHOK-1 cell lines. More development and validation in a broader range of cell lineages is required for predicting the best feed for design of a "kit".
Riesberg, J. & Yenne, S., Use of Metabolic Flux Analysis to Expedite Media Selection and Optimization for CHO Clones, NIIMBL National Meeting, Washington, DC, July 14, 2021.
Riesberg, J., Yenne, S., Shree, M., Gregory, K., Hagstrom, L., Somarriba, F., Abbott, J., Anderson, B., & Lawshe, A., Combining Multivariate Data Analysis and Metabolic Flux Analysis to Expedite Media Selection and Optimization for CHO Clones, European Society for Animal Cell Technology (ESACT), Lisbon, Portugal, June 26, 2022.
Riesberg, J. & Yenne, S., Use of Metabolic Flux Analysis (MFA) to Expedite Media Selection and Optimization for CHO Clones, NIIMBL National Meeting, Washington, DC, June 27, 2019.
Riesberg, J., Clonal Grouping of CHO cells, STL Summit, St. Louis, MO, October 3, 2019.
Use of Metabolic Flux Analysis (MFA) to Expedite Media Selection and Optimization of CHO (PC2.1-097), NIIMBL Member Forum, Virtual, March 26, 2020.
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EMD Millipore Corporation
Metalytics
